neutrophil elastase  (R&D Systems)

Journal: bioRxiv

Article Title: Multimodal profiling of proinflammatory protease activity identifies caspase-1 as a target for lung cancer interception

doi: 10.1101/2025.07.01.662195

Figure Lengend Snippet: ( A ) Immunofluorescence staining of protease protein expression (red): cathepsin G (CTSG), caspase-1, and neutrophil elastase (ELANE), in 6-week KPS tumors. All sections counterstained with DAPI (blue). Scale bar 50um. ( B ) Schematic of antibody versus activity zymography probe (AZP) staining on frozen tissue sections. Protease cleavable substrates can be converted to AZPs that show cleavage locally on tissue. AZPs consist of a negatively charged reporter masked by a peptide substrate sequence that prevents it from binding to tissue until cleaved by localized target proteases. ( C ) Binding of activated AZPs (red) cleavable by CTSG (A1), caspase-1 (A10), and ELANE (A12) in lung tumor tissue. All sections counterstained with DAPI (blue). Scale bar 50um. ( D ) A10 AZP stained 7-week KPS lung sections significantly more in tumors and less in normal adjacent tissue (NAT) or healthy tissue. All sections counterstained with DAPI (blue). Scale bar 25um. Percentage of tissue regions that were positively stained for A10 were quantified. One-way ANOVA with Tukey’s multiple comparison test was performed (p < 0.05).

Article Snippet: Recombinant proteases screened included caspase-1 (ALX-201-056-U025, Enzo), caspase-3 (707-C3, R&D Systems), caspase-7 (823-C7, R&D Systems), caspase-8 (ALX-201-163-C020, Enzo), cathepsin B (965-CY, R&D Systems), cathepsin G (BML-SE283-0100, Enzo), chymase-1 (4099-SE, R&D Systems), chymotrypsin (6907-SE, R&D Systems), granzyme B (1865-SE, R&D Systems), meprin A (4007-ZN, R&D Systems), meprin B (3300-ZN, R&D Systems), and neutrophil elastase (4517-SE, R&D Systems).

Techniques: Immunofluorescence, Staining, Expressing, Activity Assay, Zymography, Sequencing, Binding Assay, Comparison

Journal: Cell death discovery

Article Title: Highly sensitive magnetic particle imaging of abdominal aortic aneurysm NETosis with anti-Ly6G iron oxide nanoparticles.

doi: 10.1038/s41420-024-02156-3

Figure Lengend Snippet: Fig. 2 Neutrophil infiltration and NETs release within AAA lesions. A IF staining for neutrophil marker (Ly6G) was performed on tissue sections of the mouse abdominal aorta. B Hyalinization and IF labeling of the whole abdominal aorta and 3D tissue imaging were acquired with light-sheet microscopy. C–E Quantification of NETs markers (ELANE and MPO) in mouse abdominal aorta by western blotting (n = 3). **P < 0.01, ****P < 0.0001, indicating statistical significance between the two groups.

Article Snippet: Protein expression was detected using primary antibodies against ELANE (Boster Biotech, Wuhan, China), MPO (Proteintech, Wuhan, China) and GAPDH (Servicebio, Wuhan, China).

Techniques: Staining, Marker, Labeling, Imaging, Microscopy, Western Blot

Journal: PNAS Nexus

Article Title: A breath-based in vitro diagnostic assay for the detection of lower respiratory tract infections

doi: 10.1093/pnasnexus/pgae350

Figure Lengend Snippet: Evaluation of the HNE substrate sensor using rmNE and rhNE. A ) Representative MALDI-TOF mass spectra of the sensor and the cleavage products with different time points of reaction using rmNE. B ) The ratio of cleavage products to the sensor with different time points. C ) Specificity testing using a blank control, 15 µM of rhNE, 15 µM of cathepsin G (CTSG), and 15 µM of PR3. D ) LOD test at 37 °C for 24 h using different concentrations of rhNE.

Article Snippet: Recombinant mouse neutrophil elastase (rmNE) (Catalog # 4517-SE), recombinant human neutrophil elastase (rhNE) (Catalog # 9167-SE), and recombinant mouse active cathepsin C/DPPI (rmCatC, Catalog # 2336-CY) were purchased from R&D Systems (Minneapolis, MN).

Techniques: Control